Chronic Endotoxemia in Subjects with Type-1 Diabetes Is Seen Much before the Onset of Microvascular Complications

Stitt, A and Aravindhan, V and Mohan, V and Arunkumar, N and Sandhya, S and Babu, S (2015) Chronic Endotoxemia in Subjects with Type-1 Diabetes Is Seen Much before the Onset of Microvascular Complications. PLOS ONE, 10 (9). e0137618. ISSN 1932-6203



BACKGROUND: Lipopolysaccharide (LPS)/Endotoxin is hypothesized to play an important role in chronic inflammation associated with Type-1 diabetes (T1DM) and its complications. Endotoxin core antibodies (EndoCAb), LPS binding protein (LBP) and soluble CD14 (sCD14) act as modulators of LPS induced activation of innate immune system in vivo. For the present study we estimated the levels of LPS and its translocation markers in T1DM subjects with and without microvascular complications (MVC) and correlate them with clinical parameters of T1DM and serum inflammatory cytokine levels (TNF-α, IL-6, IL-1β and GM-CSF). METHODS: A total of 197 subjects (64 normal glucose tolerance (NGT) subjects, 97 T1DM subjects without MVC and 36 with MVC) were included in this study and the levels of serum LPS, its translocation markers and cytokines measured by immunoassays. RESULTS: Compared to NGT, T1DM subjects (both with and without MVC) had significantly higher levels of LPS, reduced levels of LBP and EndoCAb along with significant increase in the levels of IL-1β, IL-6, TNF-α and GM-CSF (p<0.05). No significant change was seen in the levels of these biomarkers between T1DM subjects with and without MVC. CONCLUSIONS: Decreased levels of EndoCAb and LBP suggest sustained endotoxin activity in T1DM subjects even before the onset of microvascular complications.

Item Type:Article
Official URL/DOI:
Uncontrolled Keywords:Chronic Endotoxemia; Type 1 Diabetes;
Subjects:Biochemistry,Cell and Molecular Signalling > Biochemistry Diabetes
Diabetology > Diabetes Mellitus Type 1
Divisions:Department of Diabetology
Department of Advanced Research Biochemistry
ID Code:935
Deposited By:surendar radha
Deposited On:25 Sep 2015 11:46
Last Modified:25 Sep 2015 11:46

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