Human islet mass, morphology, and survival after cryopreservation using the Edmonton protocol

Priya, M and Mohan, V and Ganthimathy, S and Anjana, R M and Gunasekaran, V and Venkatachalam, T and Thomas , A and Tatsuya, K and AM James, S and Jonathan RT, L (2013) Human islet mass, morphology, and survival after cryopreservation using the Edmonton protocol. Islets, 5 (5). ISSN 1938-2014



The aim of this study was to assess recovery, cell death, and cell composition of post-thaw cultured human islets. Cryopreserved islets were provided by the Clinical Islet Transplant Program, Edmonton, Canada. Islets were processed using media prepared in accordance with Pre-Edmonton and Edmonton protocols. Cryopreserved islets were rapidly thawed and cultured for 24 h, 3 d, 5 d, and 7 d, following which they were processed for histology. Islet quantification, integrity, morphology and tissue turnover were studied via hematoxylin and eosin stained sections. Ultrastructure was studied by electron microscopy and endocrine cell composition by immunohistochemistry. Using the Pre-Edmonton protocol, islet recovery was 50.1% and islet survival was 50% at 24 h while for the Edmonton protocol, the islet recovery was 69.4% (p<0.001) and islet survival, 50% at ≈2.5 d. With an increasing culture duration although the physical integrity was retained there was an increasing loss of cohesivity both at light microscopic and at ultrastructure level regardless of the protocols used. Percentage islet survival and tissue turnover correlated negatively with culture duration in both protocols. The Edmonton protocol appears to preserve the islets better. However, culture duration adversely affects islet survival and quality, indicating the need for more optimal cryopreservation and culture techniques.

Item Type:Article
Official URL/DOI:
Uncontrolled Keywords:clinical phase; cryopreservation; immunohistochemistry; islet isolation; islet transplantation; post-thaw culture; pre-clinical phase; ultrastructure TUNEL
Divisions:Department of Cell and Molecular Biology
Department of Diabetology
ID Code:845
Deposited By:surendar radha
Deposited On:23 Sep 2014 14:21
Last Modified:23 Sep 2014 14:21

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