Association study of the ABCC8 gene variants with type 2 diabetes in south Indians

Radha, V and Bodhini, D and Narayani, N and Mohan, V (2014) Association study of the ABCC8 gene variants with type 2 diabetes in south Indians. Indian Journal of Human Genetics, 20 (1). p. 37. ISSN 0971-6866

[img]
Preview
PDF
608Kb

Abstract

BACKGROUND: The ABCC8 gene which encodes the sulfonylurea receptor plays a major role in insulin secretion and is a potential candidate for type 2 diabetes. The ‑3c → t (rs1799854) and Thr759Thr (C → T, rs1801261) single nucleotide polymorphisms (SNPs) of the ABCC8 gene have been associated with type 2 diabetes in many populations. The present study was designed to investigate the association of these two SNPs in an Asian Indian population from south India. MATERIALS AND METHODS: A total of 1,300 subjects, 663 normal glucose tolerant (NGT) and 637 type 2 diabetic subjects were randomly selected from the Chennai Urban Rural Epidemiology Study (CURES). The ‑3c → t and Thr759Thr were genotyped in these subjects using polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP) and a few variants were confirmed by direct sequencing. RESULTS: The frequency of the ‘t’ allele of the ‑3c → t SNP was found to be 0.27 in NGT and 0.29 in type 2 diabetic subjects (P = 0.44). There was no significant difference in the genotypic frequency between the NGT and type 2 diabetic group (P = 0.18). Neither the genotypic frequency nor the allele frequency of the Thr759Thr polymorphism was found to differ significantly between the NGT and type 2 diabetic groups. CONCLUSION: The ‑3c → t and the Thr759Thr polymorphisms of the ABCC8 gene were not associated with type 2 diabetes in this study. However, an effect of these genetic variants on specific unidentified sub groups of type 2 diabetes cannot be excluded.

Item Type:Article
Official URL/DOI:http://dx.doi.org/10.4103/0971-6866.132752
Uncontrolled Keywords:ABCC8 gene, south Indians, sulfonylurea receptor, type 2 diabetes
Subjects:Genetics and Diabetes
Divisions:Department of Advanced Research Biochemistry
ID Code:812
Deposited By:surendar radha
Deposited On:25 Jul 2014 11:03
Last Modified:25 Jul 2014 11:03

Repository Staff Only: item control page