Association of His1085HisINSRGene Polymorphism with Type 2 Diabetes in South Indians

Bodhini, D and Sandhiya, M and Ghosh, S and Majumder, P P and Rao, M R S and Mohan, V and Radha, V (2012) Association of His1085HisINSRGene Polymorphism with Type 2 Diabetes in South Indians. Diabetes Technology & Therapeutics, 14 (8). p. 696. ISSN 1520-9156

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Background and Objective: The INSR gene, which encodes the insulin receptor, is a candidate gene for type 2 diabetes (T2D). The objective of the present study was to sequence some of the crucial exons in the INSR gene such as exon 2, which encodesthe insulin-binding domain of the INSR protein, and exons 17–21, which encode the protein tyrosine kinase domain for mutations/polymorphisms, and to study their association with T2D in the South Indian population. Subjects and Methods: The INSR gene was sequenced in 25 normal glucose-tolerant (NGT) and 25 T2D subjects, and the variant found was genotyped by polymerase chain reaction–restriction fragment length polymorphism in 1,016 NGT and 1,010 T2D subjects, randomly selected from the Chennai Urban Rural Epidemiology Study. Results: Only one previously reported polymorphism, His1085His [rs1799817, (C/T)], in exon 17 was detected by sequencing. The frequency of the ‘‘T’’ allele of the His1085His polymorphism was significantly lower in the T2D subjects (31%)compared with the NGT subjects (35%) and showed significant protection against diabetes (odds ratio 0.85, 95% confidence interval 0.75–0.97, P = 0.019). Regression analysis according to a recessive model taking the CC+CT genotype as the reference showed that the TT genotype was protective against diabetes (odds ratio 0.71, 95% confidence interval 0.50–0.99, P = 0.048). Adjusting this P value by the number of competing models (three) using Bonferroni’s correction, we found that the association finding did not remain significant. Conclusions: The ‘‘T’’ allele of the His1085His polymorphism in the INSR gene shows significant protection against diabetes. This study gains importance because there are no data available to date on the role of INSR variants in T2D in the Indian population.

Item Type:Article
Official URL/DOI:
Uncontrolled Keywords:CURES 90; INSR Gene; polymorphism; Type 2 Diabetes; South Indian
Subjects:Genetics and Diabetes > Genetic Risk Factors
Diabetes Clinical Trials
Divisions:Department of Epidemiology
Department of Diabetology
ID Code:764
Deposited By:surendar radha
Deposited On:17 Oct 2013 11:43
Last Modified:17 Oct 2013 11:43

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