Amelioration of Glucolipotoxicity-Induced Endoplasmic Reticulum Stress by a “Chemical Chaperone” in Human THP-1 Monocytes

Lenin, R and Maria, M S and Agrawal, M and Balasubramanyam, J and Mohan, V and Balasubramanyam, M (2012) Amelioration of Glucolipotoxicity-Induced Endoplasmic Reticulum Stress by a “Chemical Chaperone” in Human THP-1 Monocytes. Experimental Diabetes Research, 2012 . p. 1. ISSN 1687-5214

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Abstract

Chronic ER stress is emerging as a trigger that imbalances a number of systemic and arterial-wall factors and promote atherosclerosis. Macrophage apoptosis within advanced atherosclerotic lesions is also known to increase the risk of atherothrombotic disease. We hypothesize that glucolipotoxicity might mediate monocyte activation and apoptosis through ER stress. Therefore, the aims of this study are (a) to investigate whether glucolipotoxicity could impose ER stress and apoptosis in THP-1 human monocytes and (b) to investigate whether 4-Phenyl butyric acid (PBA), a chemical chaperone could resist the glucolipotoxicity-induced ER stress and apoptosis. Cells subjected to either glucolipotoxicity or tunicamycin exhibited increased ROS generation, gene and protein (PERK, GRP-78, IRE1α, and CHOP) expression of ER stress markers. In addition, these cells showed increased TRPC-6 channel expression and apoptosis as revealed by DNA damage and increased caspase-3 activity. While glucolipotoxicity/tunicamycin increased oxidative stress, ER stress, mRNA expression of TRPC-6, and programmed the THP-1 monocytes towards apoptosis, all these molecular perturbations were resisted by PBA. Since ER stress is one of the underlying causes of monocyte dysfunction in diabetes and atherosclerosis, our study emphasize that chemical chaperones such as PBA could alleviate ER stress and have potential to become novel therapeutics.

Item Type:Article
Official URL/DOI:http://dx.doi.org/10.1155/2012/356487
Uncontrolled Keywords:Glucolipotoxicity-Induced;Cell and Molecular Biology;Chemical Chaperone;Human THP-1Monocytes
Subjects:Biochemistry,Cell and Molecular Signalling
Diabetes
Divisions:Department of Cell and Molecular Biology
ID Code:619
Deposited By:INVALID USER
Deposited On:04 Jul 2012 09:55
Last Modified:04 Jul 2012 09:55

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