Plausible diagnostic value of urinary isomeric dimethylarginine ratio for diabetic nephropathy

Parmar, D and Bhattacharya, N and Kannan, S and Vadivel, S and Pandey, G K and Ghanate, A and Ragi, N C and Prabu, P and Pramodkumar, T A and Manickam, N and Mohan, V and Sripadi, P and Kuppan, G and Panchagnula, V (2020) Plausible diagnostic value of urinary isomeric dimethylarginine ratio for diabetic nephropathy. Scientific Reports, 10 (1). ISSN 2045-2322

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Abstract

Altered circulatory asymmetric and symmetric dimethylarginines have been independently reported in patients with end-stage renal failure suggesting their potential role as mediators and early biomarkers of nephropathy. These alterations can also be reflected in urine. Herein, we aimed to evaluate urinary asymmetric to symmetric dimethylarginine ratio (ASR) for early prediction of diabetic nephropathy (DN). In this cross-sectional study, individuals with impaired glucose tolerance (IGT), newly diagnosed diabetes (NDD), diabetic microalbuminuria (MIC), macroalbuminuria (MAC), and normal glucose tolerance (NGT) were recruited from Dr. Mohans' Diabetes Specialties centre, India. Urinary ASR was measured using a validated high-throughput MALDI-MS/MS method. Significantly lower ASR was observed in MIC (0.909) and MAC (0.741) in comparison to the NGT and NDD groups. On regression models, ASR was associated with MIC [OR: 0.256; 95% CI: 0.158-0.491] and MAC [OR 0.146; 95% CI: 0.071-0.292] controlled for all the available confounding factors. ROC analysis revealed ASR cut-point of 0.95 had C-statistic of 0.691 (95% CI: 0.627-0.755) to discriminate MIC from NDD with 72% sensitivity. Whereas, an ASR cut-point of 0.82 had C-statistic of 0.846 (95% CI: 0.800 - 0.893) had 91% sensitivity for identifying MAC. Our results suggest ASR as a potential early diagnostic biomarker for DN among the Asian Indians.

Item Type:Article
Official URL/DOI:http://dx.doi.org/10.1038/s41598-020-59897-1
Uncontrolled Keywords:diabetic nephropathy
Subjects:Biochemistry,Cell and Molecular Signalling
Divisions:Department of Advanced Research Biochemistry
ID Code:1229
Deposited By:surendar radha
Deposited On:04 Aug 2021 12:21
Last Modified:04 Aug 2021 12:21

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